Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Salmonella Enteritidis activates inflammatory storm via SPI-1 and SPI-2 to promote intracellular proliferation and bacterial virulence

doi: 10.3389/fcimb.2023.1158888

Figure Lengend Snippet: Construction of S. Enteritidis ΔSPI-1 , ΔSPI-2 and ΔSPI-1/SPI-2 deficient mutants and biochemical identification of the deficient strains. (A) Schematic representation of the construction for S. Enteritidis ΔSPI-1 , ΔSPI-2 and ΔSPI-1/SPI-2 mutants. The Salmonella deficient mutants were produced by using the λ-Red recombinase gene replacement method. (B) All gene deletions were verified by PCR analysis and by sequencing. The stn gene was used as the reference control. (C) Growth curves of S. Enteritidis C50041 wild-type, ΔSPI-1 , ΔSPI-2 and ΔSPI-1/SPI-2 deletion strains. Bacteria were cultured in LB medium at 37°C with 180 rpm, and the OD 600 values of bacterial cultures were determined in 0.5 h intervals. (D) Biochemical tests of S. Enteritidis WT, ΔSPI-1 , ΔSPI-2 and ΔSPI-1/SPI-2 strains using the API 20E identification kit. Sterile water was used as control.

Article Snippet: Biochemical tests were performed using an API 20E identification kit (bioMerieux SA, Lyon, France) in accordance with the manufacturer’s protocol.

Techniques: Produced, Sequencing, Control, Bacteria, Cell Culture, Sterility